Keynote speaker: Riccardo Olcese, PhD, Professor of Anesthesiology and Physiology
Director, Basic Science Research Program Administration, Department of Anesthesiology & Perioperative Medicine, Division of Molecular Medicine BH 569 CHS, David Geffen School of Medicine, University of California Los Angeles
Title: “The Voltage Sensors that Govern CaV1.1 Channel Opening and Skeletal Muscle Contraction”
Summary: Initiation of skeletal muscle contraction is triggered by rapid activation of RyR1 channels in response to sarcolemmal depolarization. RyR1 is an intracellular channel and has no voltage-sensing structures, but it is coupled with the voltage-sensing apparatus of CaV1.1 channels to inherit voltage sensitivity. Using an opto-electrophysiological approach, the Olcese laboratory at the University of California Los Angeles studies the excitation-driven molecular events controlling both CaV1.1 pore opening and RyR1 activation. Each of the four CaV1.1 voltage-sensing domains exhibits unique biophysical properties in terms of kinetics and voltage dependence, providing a rationale for how CaV1.1, a slowly-activating channel, can trigger the rapid activation of RyR1.
Keynote lecture
10.00-11.00 The Voltage Sensors that Govern CaV1.1 Channel Opening and Skeletal Muscle Contraction
Riccardo Olcese (University of California Los Angeles, USA)
Oral communications (morning session)
11.00-11.15 A corneal neuralgia TRPV1 mutation increases response to acidic pH and alters agonist sensitization and desensitization
Roberta Gualdani, Philippe Gailly, Xavier Yerna, Solène Barbeau, Deborah S. Jacobs, Sulayman Dib-Hajj, Stephen G. Waxman (UCLouvain, Massachusetts Eye and Ear, and Yale School of Medicine, USA)
11.15-11.30 The glycolytic enzyme pyruvate kinase M2 (PKM2) functions as a modulator of cytosolic and mitochondrial Ca2+ signaling in a cancer cell line
Fernanda O. Lemos, Ian de Ridder, Shreya Ramesh, Martin D. Bootman, Geert Bultynck, Jan B. Parys (KULeuven, and The Open University, Walton Hall, UK)
11.30-11.45 Unraveling the impact of intracellular BAPTA on cell function and the development of novel intracellular Ca2+ chelators
Femke Speelman-Rooms, Flore Sneyers, Martin Bootman, Geert Bultynck, Steven Verhelst (KULeuven)
11.45-12.00 Selection of peptides for a muscle-targeted delivery of ASO directed against DUX4 mRNAs through complementary approaches in silico, in vitro and in vivo
Maëlle Limpens, Aline Derenne, Carmen Burtea, Sophie Laurent, Alexandre Legrand, Steve Wilton, Alexandra Belayew, Frédérique Coppée, Anne-Emilie Declèves, Alexandra Tassin (UMons)
12.00 – 12.30 Lunch
12.30 – 13.30 Guided Poster Session
Posters:
• Molecular mechanisms of aneuploidy and glioblastoma aggressiveness induced by Diaph3 loss
Caren Jabbour, Nicolas Tajeddine, Philippe Gailly, Roberta Gualdani, Olivier Schakman, Farah Issa, Thibaud Parpaite, Xavier Yerna, Solène Barbeau (UCLouvain)
• A Not-So-Simple Simplification
Romain Vitello, Hossein Taouba, Donna Pereira Barbon, Nikolay Tumanov, Johan Wouters, Vincent Seutin, Jean-François Liégeois (ULiège and UNamur)
• Silica nanoparticles inhibit responses to ATP in human airway epithelial 16HBE cells
Alina Milici, Alicia Sanchez, Karel Talavera (KULeuven)
• A theoretical framework for polymodal gating of sensory TRP channels
Inti Zumeta-Dubé, Alina Milici, Enrique Velasco, Karel Talavera (KULeuven)
• Modulation of the chemosensory cation channel TRPA1 by cholesterol reducing drugs.
Justyna B. Startek, Alina Milici, Katharina Held, Thomas Voets, Karel Talavera (KULeuven)
Oral communications (afternoon session)
13.30-13.45 Sustained Intermittent Hypoxaemia as a component of COPD pathophysiology: which effect on skeletal muscle?
Lise Paprzycki, Yamina Gourari, Alexandre Legrand, Florence Debacq-Chainiaux, Alexandra Tassin (UMons, UNamur)
13.45-14.00 Serpina3: a novel marker of anthracycline-induced cardiovascular toxicity: from bench to bedside
Dustin Krüger, Matthias Bosman, Hanne Boen, Emeline Van Craenenbroeck, Constantijn Franssen, Pieter-Jan Guns, Charles-Henri Van Assche, Berta Cillero, Leen Delrue, Ward Heggermont, Guido De Meyer (UAntwerp, UZA, M4i Maastricht, OLV Aalst)
14.00-14.15 Conditional deletion of KCC2 impairs synaptic plasticity and both spatial and nonspatial memory
A Kreis, F Issa, X Yerna, C Jabbour, O Schakman, M de Clippele, N Tajeddine, N Pierrot, JN Octave, R Gualdani, P Gailly (UCLouvain)
14.15-14.30 Role of subthreshold membrane potential instabilities in sensory transduction
Enrique Velasco, Julio L. Alvarez, Karel Talavera (KULeuven)
14.30-15:00 Coffee – Tea and Networking
Closing lecture
15.00-15.45 Cytosolic Sodium Keeps a Tight Rein on the Na+-Ca2+ Exchanger to Tune Cardiac Function
Michela Ottolia (University of California Los Angeles, USA)
